First Ever Whole Genome Sequencing and De Novo Assembly of the Freshwater Angelfish, Pterophyllum scalare

Indeever Madireddy

doi:10.17912/micropub.biology.000654

First Ever Whole Genome Sequencing and De Novo Assembly of the Freshwater Angelfish, Pterophyllum scalare

MicroPubl Biol. 2022 Oct 18:2022:10.17912/micropub.biology.000654. doi: 10.17912/micropub.biology.000654. eCollection 2022.

Author

Indeever Madireddy^{1

2}

Affiliations

¹ BioCurious, Santa Clara, CA, USA.
² BASIS Independent Silicon Valley, San Jose, CA, USA.

Abstract

This research work is the first to ever sequence and perform a de novo assembly of the genome of the freshwater angelfish, Pterophyllum scalare . The final genome assembly consisted of 15,486 contigs and was 734.79 Mb in size with an 86.5% BUSCO score. Functional annotation of the genome revealed 24,247 protein-coding sequences related to other fish species. 14,329 (59%) of the identified genes were orthologous to Archocentrus centrarchus , a closely related South American cichlid.