SARS-CoV-2 protease Nsp3 is a therapeutic target for developing anti-SARS-CoV-2 drugs. Nsp3 is a large multi-spanning membrane protein, and its characterization in vitro has been challenging. Here we describe an in vitro assay to characterize the biochemical activity of full-length Nsp3 isolated from cells. The assay can be used to evaluate Nsp3 inhibitors.
Keywords: Deubiquitinase; ISG15-CHOP2; Nsp3; PLpro; SARS-CoV-2; Ub-Rh110; Ubiquitin; deISGylase.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.