Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines

Yuta Fukawa; Keita Yoshida; Sayaka Degura; Koichi Mitsukura; Toyokazu Yoshida

doi:10.1039/d2cc05116h

Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines

Chem Commun (Camb). 2022 Nov 29;58(95):13222-13225. doi: 10.1039/d2cc05116h.

Authors

Yuta Fukawa¹, Keita Yoshida², Sayaka Degura², Koichi Mitsukura³, Toyokazu Yoshida³

Affiliations

¹ Department of Engineering Science, Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
² Department of Life Science and Chemistry, Graduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
³ Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. mitukura@gifu-u.ac.jp.

PMID: 36353944
DOI: 10.1039/d2cc05116h

Abstract

An engineered (S)-selective imine reductase from Streptomyces sp. GF3546 (SIR46) showed twice the activity of the wild type and high thermostability at 50 °C. Using a biocatalyst based on SIR46, we investigated the activity toward high-concentration 2-methyl-1-pyrroline up to 500 mM, conversion rates of other imines, and reductive amination activity.

MeSH terms

Amination
Amines
Imines*
Oxidoreductases / metabolism
Stereoisomerism
Streptomyces* / metabolism

Substances

Imines
Oxidoreductases
Amines