Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Methods Mol Biol. 2023:2598:65-73. doi: 10.1007/978-1-0716-2839-3_6.


Bone marrow-derived mesenchymal stromal cells (BM-MSC) are widely studied in the field of cartilage regeneration due to their capacity to differentiate into chondrocytes under specific in vitro culture conditions. This chapter describes the isolation of MSC from bone marrow aspirate, their expansion in monolayer, and the chondrogenic differentiation in pellet culture.

Keywords: Bone marrow; Chondrogenesis; MSC; Pellet culture; TGF-β1.

MeSH terms

  • Bone Marrow Cells
  • Bone Marrow*
  • Cell Differentiation
  • Cells, Cultured
  • Chondrocytes
  • Chondrogenesis
  • Humans
  • Mesenchymal Stem Cells*