The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

Methods Mol Biol. 2023:2598:115-121. doi: 10.1007/978-1-0716-2839-3_9.


The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

Keywords: Cartilage; DMMB; Dimethylmethylene blue assay; Sulfated glycosaminoglycans; sGAG.

MeSH terms

  • Glycosaminoglycans*
  • Methylene Blue*
  • Spectrophotometry


  • A73025
  • dimethylmethylene blue
  • Glycosaminoglycans
  • Methylene Blue