Osteochondral Explant Isolation and Culture Under a Compression and Shear Bioreactor

Methods Mol Biol. 2023:2598:325-336. doi: 10.1007/978-1-0716-2839-3_23.


Osteochondral explants harvested from different species are valuable preclinical ex vivo models for tissue engineering research. In this chapter, we describe the isolation of osteochondral plugs from bovine stifle joints, followed by defect creation, and plug preparation in a straightforward manner before mechanical loading using a compression and shear bioreactor. The method can be adapted to isolate osteochondral plugs from any animal species and to load explants in any type of bioreactor.

Keywords: Articular cartilage; Bioreactor; Ex vivo model; Osteochondral defects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bioreactors
  • Cartilage, Articular*
  • Cattle
  • Chondrocytes
  • Knee Joint
  • Tissue Engineering / methods