Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

Miłosz Pastuszczak; Jadwiga Stanek-Tarkowska; Miroslava Kačániová

doi:10.3390/ijerph192113721

Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

Int J Environ Res Public Health. 2022 Oct 22;19(21):13721. doi: 10.3390/ijerph192113721.

Authors

Miłosz Pastuszczak¹, Jadwiga Stanek-Tarkowska¹, Miroslava Kačániová^{2

3}

Affiliations

¹ Department of Soil Science, Environmental Chemistry and Hydrology, University of Rzeszow, 35-601 Rzeszów, Poland.
² Department of Bioenergetics, Food Analysis and Microbiology, Institute of Food and Nutrition Technology, University of Rzeszow, 35-959 Rzeszow, Poland.
³ Faculty of Horticulture and Landscape Engineering, Institute of Horticulture, Slovak University of Agriculture, 94976 Nitra, Slovakia.

Abstract

This study investigated the effect of different doses of fertilization with biomass combustion ash (Salix viminalis L. willow) on changes in the biological, chemical, and physical properties of soil. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (fertilization dose: control (without fertilization), NPK (nitrogen (N), phosphorus (P) and potassium (K)), 100, 200, 300, 400, 500 kg K₂O ha^-1). The biomass ash was characterized by a pH value of 12.83 ± 0.68 and a high content of macronutrients. The samples were collected from 0-5, 10-15, and 20-25 cm soil layers under the cultivation of spring barley (Hordeum vulgare L) cv. Planet in April and August 2021. Mass spectrometry (MALDI-TOF MS) was used for microbiological analyses, which revealed the presence of 53 culturable species from 11 genera: Bacillus, Pseudomonas, Paenibacillus, Lysinibacillus, Pseudarthrobacter, Arthrobacter, Staphylococcus, Paenarthrobacter, Micrococcus, Rhodococcus, and Flavobacterium. The podzolic and chernozem soils exhibited the presence of 28 and 44 culturable species, respectively. The study showed an increase in the number of microorganisms in the top layer of the soil profile. However, the number of bacteria decreased at the depths of 10-15 cm and 20-25 cm. With depth, the bulk density (BD) and moisture increased.

Keywords: MALDI-TOF MS Biotyper; biomass ash; bulk density (BD); culturable microbial diversity; fertilization; soil bacteria; soil moisture.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria
Biomass
Fertilization
Fertilizers / analysis
Hordeum*
Nitrogen / analysis
Phosphorus / analysis
Soil Microbiology
Soil* / chemistry

Substances

Soil
Phosphorus
Nitrogen
Fertilizers

Grants and funding

The research (J.S.-T.; M.P.) on soil material was funded within a subsidy to maintain the research potential of the Department of Soil Science, Environmental Chemistry and Hydrology of the University of Rzeszów for BS/2018/2019; BS/2020/2021.