Cellular protein turnover-the net result of protein synthesis and degradation-is crucial to maintain protein homeostasis and cellular function under steady-state conditions and to enable cells to remodel their proteomes upon a perturbation. In brain cells, proteins are continuously turned over at different rates depending on various factors including cell type, subcellular localization, cellular environment, and neuronal activity. Here we describe a workflow for the analysis of protein synthesis, degradation, and turnover in primary cultured rat neurons and glia using dynamic/pulsed SILAC and mass spectrometry.
Keywords: Dynamic SILAC; Glia; Mass spectrometry; Neurons; Protein degradation; Protein synthesis; Protein turnover; SILAC.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.