pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

So-Fong Cam Ngan; Neil E McCarthy; Siu Kwan Sze

doi:10.1007/978-1-0716-2863-8_4

pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles

Methods Mol Biol. 2023:2603:43-58. doi: 10.1007/978-1-0716-2863-8_4.

Authors

So-Fong Cam Ngan¹, Neil E McCarthy², Siu Kwan Sze³

Affiliations

¹ Department of Health Sciences, Brock University, St Catharines, ON, Canada.
² Centre for Immunobiology, The Blizard Institute, Bart's and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
³ Department of Health Sciences, Brock University, St Catharines, ON, Canada. nsze@brocku.ca.

PMID: 36370269
DOI: 10.1007/978-1-0716-2863-8_4

Abstract

The protein cargo of extracellular vesicles (EVs) determines their impact on recipient cell types and the downstream effects on biological function. Environmental cues can modify EV loading with proteins derived from the plasma membrane via endocytosis, obtained from the preexisting cytosolic pool via active sorting, or packaging with newly synthesized proteins drawn from trans-golgi networks. Given the major impact these pathways exert on EV content and functional potential, it is important to study how defined stimuli influence protein sorting into these vesicles for dispersal. To this end, pSILAC-based approaches can be used to pulse/trace the origins of EV protein content and thereby provide valuable insight into vesicle biology and likely effects on intercellular communication in diverse settings.

Keywords: Extracellular vesicles; Intercellular communication; Protein cargo; Protein sorting; pSILAC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Communication
Endocytosis
Extracellular Vesicles* / metabolism
Protein Transport
Proteins / metabolism

Substances

Proteins