SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands; Hamze Beati; H- Arno J Müller

doi:10.1007/978-1-0716-2863-8_15

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Methods Mol Biol. 2023:2603:187-198. doi: 10.1007/978-1-0716-2863-8_15.

Authors

Alistair Langlands¹, Hamze Beati², H- Arno J Müller³

Affiliations

¹ National Phenotypic Screening Centre, School of Life Sciences, University of Dundee, Dundee, UK.
² Developmental Genetics Group, Institute for Biology, University of Kassel, Kassel, Germany.
³ Developmental Genetics Group, Institute for Biology, University of Kassel, Kassel, Germany. h.a.muller@uni-kassel.de.

PMID: 36370280
DOI: 10.1007/978-1-0716-2863-8_15

Abstract

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Keywords: Development; Drosophila; Embryo; Posttranslational modification; Proteomics; SILAC.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drosophila
Drosophila melanogaster* / genetics
Isotope Labeling / methods
Protein Processing, Post-Translational
Proteomics* / methods

Abstract

Publication types

MeSH terms

Grants and funding