Generation of Bone Marrow Chimeras

Methods Mol Biol. 2023;2580:165-171. doi: 10.1007/978-1-0716-2740-2_9.


Bone marrow chimeras are widely used in immunological studies, to dissect the contributions of hematopoietic and non-hematopoietic cells in immune cell development or functions, to quantify the impact of a given mutation, or in preclinical studies for hematopoietic stem cell transplantation. Here we describe a set of procedures for the generation of bone marrow chimeras.

Keywords: Bone marrow chimeras; Hematopoietic cells; Immune reconstitution; T cell development.

MeSH terms

  • Bone Marrow Transplantation
  • Bone Marrow*
  • Cell Differentiation
  • Hematopoietic Stem Cell Transplantation*