Super-resolution re-scan second harmonic generation microscopy

Proc Natl Acad Sci U S A. 2022 Nov 22;119(47):e2214662119. doi: 10.1073/pnas.2214662119. Epub 2022 Nov 14.


Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.

Keywords: second harmonic generation; super-resolved optical imaging; two-photon excited fluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collagen
  • Microscopy, Fluorescence / methods
  • Photons
  • Radionuclide Imaging
  • Second Harmonic Generation Microscopy* / methods


  • Collagen