TRIM11, a new target of p53, facilitates the migration and invasion of nasopharyngeal carcinoma cells

Mol Biol Rep. 2023 Jan;50(1):731-737. doi: 10.1007/s11033-022-07833-z. Epub 2022 Nov 14.

Abstract

Background: Although tripartite motif-containing protein 11 (TRIM11) is known to be associated with a variety of cancers, its role in nasopharyngeal carcinoma (NPC) is unclear.

Methods and results: To investigate the role of TRIM11 in NPC, TRIM11 was stably overexpressed in 6-10B and CNE2 cells with lentiviral vectors and knocked down in S18 and 5-8F cells using the CRISPR/Cas9 system. Transwell assays and wound-healing assays revealed that TRIM11 facilitated the migration and invasion of NPC cells. Mechanistically, we found that p53 inhibits TRIM11 expression by binding to its promoter.

Conclusions: TRIM11 may serve as a potential diagnostic marker for NPC and has a certain therapeutic value.

Keywords: Invasion; Migration; Nasopharyngeal carcinoma; TRIM11; p53.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Nasopharyngeal Carcinoma / genetics
  • Nasopharyngeal Neoplasms* / genetics
  • Nasopharyngeal Neoplasms* / pathology
  • Tripartite Motif Proteins / genetics
  • Tripartite Motif Proteins / metabolism
  • Tumor Suppressor Protein p53* / genetics
  • Tumor Suppressor Protein p53* / metabolism
  • Ubiquitin-Protein Ligases / genetics
  • Ubiquitin-Protein Ligases / metabolism

Substances

  • Tumor Suppressor Protein p53
  • TRIM11 protein, human
  • Tripartite Motif Proteins
  • Ubiquitin-Protein Ligases