A comparison of mining methods to extract novel bacteriocins from Lactiplantibacillus plantarum NWAFU-BIO-BS29

Mohamedelfatieh Ismael; Tao Wang; Fangfang Yue; Yanlong Cui; Qin Yantin; Nageena Qayyum; Xin Lü

doi:10.1016/j.ab.2022.114938

A comparison of mining methods to extract novel bacteriocins from Lactiplantibacillus plantarum NWAFU-BIO-BS29

Anal Biochem. 2023 Jan 15:661:114938. doi: 10.1016/j.ab.2022.114938. Epub 2022 Nov 13.

Authors

Mohamedelfatieh Ismael¹, Tao Wang², Fangfang Yue³, Yanlong Cui⁴, Qin Yantin⁵, Nageena Qayyum⁶, Xin Lü⁷

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China; Sudanese Standard and Metrology Organization, Khartoum, 13573, Sudan. Electronic address: alfagold2008@windowslive.com.
² College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: Wtao_2017@163.com.
³ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: 462034927@qq.com.
⁴ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: 1024923026@qq.com.
⁵ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: 364611964@qq.com.
⁶ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: nageena@nwafu.edu.cn.
⁷ College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China. Electronic address: xinlu@nwsuaf.edu.cn.

PMID: 36379249
DOI: 10.1016/j.ab.2022.114938

Abstract

One of the most important challenges in the field of food safety is producing natural and safe substances that act against pathogens in food. Bacteriocins and antimicrobial peptides (AMPs) have an anti-pathogens effect for both Gram-negative and positive bacteria. The aim of this study was to isolate and characterize safe lactic acid bacteria from traditional Chinese fermented milk that can produce anti-bacterial molecule compounds and does not harm for humans and animals. Lactiplantibacillus plantarum NWAFU-BIO-BS29 was found to be safe, lacking 16 genes for virulence factors, biogenic amine production and antibiotic resistance, and no hemolysis activity was observed. In contrast, it has ability to produce a novel potential bacteriocin of Plantaricin Bio-LP1. Precipitation of bacteriocin by Ethyl-acetate proved to be a suitable method for the extraction the bacteriocin. Whilst, the purification steps were performed as follows: the protein purification system (AKTA-Purifier equipped with HiTrap (gel column)), followed by reversed phase high-performance liquid chromatography (RP-HPLC) equipped with C18 column. In addition, LC-MS-MS and MALDI-TOF were used to identify the peptide sequences and estimate the molecular weight, respectively. Notably, among the eight peptide sequences considered, a couple of sequences have been announced as uncharacterized in protein database (FDYYFFDKK and KEIDDNSIAVK) with a molecular mass less than 1.3 kDa. The MIC was 0.552 mg/ml and exhibited high stability under various temperature, pH, and enzymes conditions. The best activity was found at temperature and pH of 4 °C and 6 °C, respectively, which are the optimal conditions for preservation of most foods. We concluded that, the described method can arouse a growing interest in mining novel bacteriocins. Plantaricin Bio-LP1 is a potentially unique bacteriocin that is effective as a bio-preservative and could make a promising contribution in food and animal feed industries or in the medical field with further clinical studies.

Keywords: Antimicrobial peptides; Bacteriocin; Food safety; Inhibition zone; Lactiplantibacillus plantarum; Molecular docking.

MeSH terms

Bacteriocins* / pharmacology
Humans
Peptides

Substances

Bacteriocins
Peptides