Age-related histopathological and biochemical testicular damages were ameliorated by vitamin C administration

Rev Int Androl. 2023 Apr-Jun;21(2):100327. doi: 10.1016/j.androl.2021.10.001. Epub 2022 Nov 14.


Introduction and objectives: Aging is an irreversible process associated with decreased biological functions that can lead to the reduction of reproductive organs capacities in males and females. Paternal age is a significant predictor of offspring health and development. So, the aim of this study was to evaluate the effects of vitamin C on histopathological and biochemical testicular changes following aging process with a focus on stereological methods.

Material and methods: For this study, 48 adult male NMRI mice were divided into two control and experimental groups. Mice in experimental group were supplemented with vitamin C (150mg/kg) including 24-h interval by oral gavage for 33 weeks. Same regime was performed for animals in control group except that vitamin C was replaced by water. Then, right testes were extracted for stereological and left testes were used for molecular analyses on weeks 8, 12, and 33.

Results: Our findings showed low semen quality, decreased level of serum Luteinizing hormone (LH), Follicle-stimulating hormone (FSH), and testosterone along with increased reactive oxygen species (ROS) production and higher apoptotic gene expression following aging. Stereological studies showed that the volume of testes, the length of seminiferous tubules, and the number of spermatogenic and none-spermatogenic cells decreased significantly during aging. Also, vitamin C consumption for 33 weeks significantly improved biochemical and histological indices. The impact of aging on male reproduction seems to be inevitable worldwide. Therefore, the use of protective and preventive remedies conserving male fecundity is very important and based on our results, vitamin C is a beneficial candidate for improving age-related testicular changes due to aging process.

Keywords: Aging; Daños testiculares; Envejecimiento; Estereología; Mice; Ratones; Stereology; Testicular damages; Vitamin C; Vitamina C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascorbic Acid* / metabolism
  • Ascorbic Acid* / pharmacology
  • Female
  • Male
  • Mice
  • Semen Analysis
  • Seminiferous Tubules / pathology
  • Testis*
  • Testosterone


  • Ascorbic Acid
  • Testosterone