Endoscopic imaging of white matter fiber tracts using polarization-sensitive optical coherence tomography

Neuroimage. 2022 Dec 1;264:119755. doi: 10.1016/j.neuroimage.2022.119755. Epub 2022 Nov 15.


Polarization sensitive optical coherence tomography (PSOCT) has been shown to image and delineate white matter fibers in a label-free manner by revealing optical birefringence within the myelin sheath using a microscope setup. In this proof-of-concept study, we adapt recent advancements in endoscopic PSOCT to perform depth-resolved imaging of white matter structures deep inside intact porcine brain tissue ex-vivo, through a small, rotational fiber probe. The probe geometry is comparable to microelectrodes currently used in neurosurgical interventions. The presented imaging system is mobile, robust, and uses biologically safe levels of optical radiation making it well suited for clinical translation. In neurosurgery, where accuracy is imperative, endoscopic PSOCT through a narrow-gauge fiber probe could provide intra-operative feedback on the location of critical white matter structures.

Keywords: Birefringence; Intracranial imaging; Optical anisotropy; Optical coherence tomography; Polarized light imaging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Birefringence
  • Brain / diagnostic imaging
  • Myelin Sheath
  • Swine
  • Tomography, Optical Coherence* / methods
  • White Matter* / diagnostic imaging