Generation of integration-free induced pluripotent stem cell line (KSCBi017-A) from peripheral blood mononuclear cells of a healthy male individual

Young Sam Im; Dae Hoon Yoo; Hyung-Eun Kim; Ji Young Oh; Yong-Ou Kim

doi:10.1016/j.scr.2022.102965

Generation of integration-free induced pluripotent stem cell line (KSCBi017-A) from peripheral blood mononuclear cells of a healthy male individual

Stem Cell Res. 2022 Dec:65:102965. doi: 10.1016/j.scr.2022.102965. Epub 2022 Nov 11.

Authors

Young Sam Im¹, Dae Hoon Yoo¹, Hyung-Eun Kim¹, Ji Young Oh¹, Yong-Ou Kim²

Affiliations

¹ Division of Intractable Diseases Research, Korea National Institute of Health, Osong, Cheongju-si, South Korea.
² Division of Intractable Diseases Research, Korea National Institute of Health, Osong, Cheongju-si, South Korea. Electronic address: youkim@korea.kr.

PMID: 36403547
DOI: 10.1016/j.scr.2022.102965

Abstract

A human induced pluripotent stem cell (hiPSC) line, KSCBi017-A, was generated from a 50-year-old male individual using non-integrating episomal vectors expressing reprogramming factors. The generated hiPSCs were integration-free, expressed pluripotency markers, exhibited the potential for differentiation into three germ layers in vivo, and maintained the normal karyotype. This cell line can be used as a control for a disease model and is available from Korea National Stem Cell Bank.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male
Middle Aged