Protective Effects of Coenzyme Q10 Along with Fe2O3 Nanoparticles On Sperm Parameters in Rats with Scrotal Hyperthermia: Effects of CoQ 10 and Fe2O3 Nanoparticles On Sperm Parameters

Mahshid Deldar Abad Paskeh; Nahid Babaei; Maliheh Entezari; Mehrdad Hashemi; Abbas Doosti

doi:10.31661/gmj.v11i.2046

Protective Effects of Coenzyme Q10 Along with Fe₂O₃ Nanoparticles On Sperm Parameters in Rats with Scrotal Hyperthermia: Effects of CoQ 10 and Fe₂O₃ Nanoparticles On Sperm Parameters

Galen Med J. 2022 Aug 27:11:1-7. doi: 10.31661/gmj.v11i.2046. eCollection 2022.

Authors

Mahshid Deldar Abad Paskeh¹, Nahid Babaei¹, Maliheh Entezari^{1

2}, Mehrdad Hashemi^{2

3}, Abbas Doosti⁴

Affiliations

¹ Department of Cell Biology and Genetics, Bushehr Branch, Islamic Azad University, Bushehr, Iran.
² Farhikhtegan Medical Convergence sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
³ Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁴ Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Abstract

Background: One of the most important factors in reducing the birth rate is male infertility, and one of the main reasons for male infertility is scrotal hyperthermia (SH). Therefore, this study aimed to investigate the protective effect of coenzyme Q10 (CoQ10) along with Fe₂O₃ nanoparticles on semen parameters in rats with SH. Materials and Methods: Forty-eight adult male Wistar rats were divided into eight groups: healthy control, control group receiving Fe₂O₃ nanoparticles, control group receiving CoQ10, control group receiving Fe₂O₃ nanoparticles plus CoQ10, SH group, SH group receiving CoQ10, SH group receiving Fe₂O₃ nanoparticle, and SH group receiving Fe₂O₃ nanoparticles plus CoQ10. After killing rats, semen was collected from epididymal tissue, and parameters such as sperm viability, motility, concentration, and morphology were studied. Results: SH significantly reduced sperm concentration, motility, and viability, as well as altering sperm morphology in rats. Nevertheless, CoQ10 strongly improved sperm parameters in SH rats. Fe₂O₃ nanoparticles led to a sharp decrease in sperm parameters; however, during the simultaneous administration of Fe₂O₃ nanoparticles with CoQ10, improvement in sperm parameters was seen in the SH rats. Conclusion: Our findings suggest that CoQ10, along with Fe₂O₃ nanoparticles, has a protective effect against spermatogenic cell death induced by SH. Thus, green synthesis of nanoparticles with the administration of antioxidants, including CoQ10 is recommended for the treatment of SH.

Keywords: CoQ10; Fe2O3; Nanoparticle; Scrotal Hyperthermia; Sperm.