The PGE2/Ptger4b pathway regulates ovulation by inducing intracellular actin cytoskeleton rearrangement via the Rho/Rock pathway in the granulosa cells of periovulatory follicles in the teleost medaka

Katsueki Ogiwara; Chika Fujimori; Takayuki Takahashi

doi:10.1016/j.mce.2022.111816

The PGE₂/Ptger4b pathway regulates ovulation by inducing intracellular actin cytoskeleton rearrangement via the Rho/Rock pathway in the granulosa cells of periovulatory follicles in the teleost medaka

Mol Cell Endocrinol. 2023 Jan 15:560:111816. doi: 10.1016/j.mce.2022.111816. Epub 2022 Nov 19.

Authors

Katsueki Ogiwara¹, Chika Fujimori², Takayuki Takahashi²

Affiliations

¹ Laboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan. Electronic address: kogi@sci.hokudai.ac.jp.
² Laboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan.

PMID: 36410550
DOI: 10.1016/j.mce.2022.111816

Abstract

We have previously shown that the prostaglandin E₂/Ptger4b receptor system is involved in ovulation in teleost medaka and induces intracellular actin cytoskeleton rearrangement in the granulosa cells of preovulatory follicles. In this study, we investigated the signaling pathways through which prostaglandin E₂ induces a change in the actin cytoskeleton. Treating preovulatory follicles with GW627368X (Ptger4b antagonist), a Rho inhibitor, or Y-27632 [Rho-associated protein kinase (Rock) inhibitor] inhibited not only in vitro follicle ovulation but also intracellular actin cytoskeleton rearrangement. Active Rhoa-c and Rock1 were detected in follicles immediately before ovulation. GW627368X also inhibited Rhoa-c activation and cytoskeleton rearrangement. PGE₂-induced actin cytoskeleton rearrangement was not observed in the Ptger4b-, Rhoa-c-, or Rock1-deficient OLHNI-2 cells. These results indicate that the PGE₂/Ptger4b pathway regulates intracellular actin cytoskeleton rearrangement via the Rho/Rock pathway in the granulosa cells of preovulatory follicles during medaka ovulation.

Keywords: Intracellular actin cytoskeleton rearrangement; Medaka ovulation; PGE(2); Ptger4b; Rho; Rock.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton
Animals
Dinoprostone
Female
Granulosa Cells
Oryzias*
Ovulation / physiology
rho-Associated Kinases

Substances

N-(2-(4-(4,9-diethoxy-1-oxo-1,3-dihydro-2H-benzo(f)isoindol-2-yl)phenyl)acetyl)benzene sulphonamide
Dinoprostone
rho-Associated Kinases