Inhibition of the Exocyst Complex with Endosidin 2 Reduces Polarized Growth in Physcomitrium patens

MicroPubl Biol. 2022 Nov 4:2022:10.17912/micropub.biology.000655. doi: 10.17912/micropub.biology.000655. eCollection 2022.

Abstract

Endosidin 2 (ES2) is a cell-permeable drug that binds to the Exo70 subunit of the exocyst complex, disrupting the final stages of exocytosis. This allows for a dose-dependent control over the process of exocytosis and greater ease in studying exocytic-dependent processes such as polarized cell growth. ES2 was utilized in studying polarized cell growth in the moss Physcomitrium patens , in which plants were exposed to increasing concentrations of ES2 with an IC50 between 8.8 and 12.3 µM. At 50 µM, tip-growing cells ruptured close to their tips, an indication that ES2 inhibits the deposition of new cell wall material via exocytosis. This data serves to further support the use of ES2 as a tool to interfere with exocytosis with lethality only seen at high levels of ES2.