Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence

F1000Res. 2022 Aug 24;11:977. doi: 10.12688/f1000research.124632.2. eCollection 2022.

Abstract

TBK1 is a serine-threonine protein kinase that has been linked to a number of diseases including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for TBK1 for use in immunoblot, immunofluorescence and immunoprecipitation, using an isogeneic knock-out cell line as a control. We identify antibodies that appear specific for all three applications but invite the readers to interpret the present findings based on their own scientific expertise and use this report as a guide to select the most appropriate antibody for their specific needs.

Keywords: TBK1; Uniprot# Q9UHD2; Western blot; antibody characterization; antibody validation; immunoblot; immunofluorescence; immunoprecipitation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Antibody Technique
  • Immunoprecipitation
  • Mutation
  • Protein Serine-Threonine Kinases*
  • Serine
  • Threonine*

Substances

  • Protein Serine-Threonine Kinases
  • Threonine
  • Serine