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. 2022 Dec 8;387(23):2194-2196.
doi: 10.1056/NEJMc2212772. Epub 2022 Nov 23.

Neutralization of Omicron Subvariant BA.2.75 after Bivalent Vaccination

Spyros Chalkias  1 , Jing Feng  1 , Xing Chen  1 , Honghong Zhou  1 , Jean-Claude Marshall  1 , Bethany Girard  1 , Joanne E Tomassini  1 , Barbara J Kuter  1 , David C Montefiori  2 , Rituparna Das  3
Affiliations

Neutralization of Omicron Subvariant BA.2.75 after Bivalent Vaccination

Spyros Chalkias et al. N Engl J Med. .
No abstract available

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Figures

Figure 1
Figure 1. Neutralization of the Ancestral SARS-CoV-2 D614G Strain and Omicron Subvariants after Receipt of mRNA-1273.214 as a Second Booster Dose.
Pseudovirus neutralizing antibody titers against the ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) D614G strain and the omicron subvariants BA.1, BA.4 and BA.5 (these subvariants have identical sequences and were therefore designated as a composite), and BA.2.75 were assessed in serum samples from adult participants in the per-protocol immunogenicity population who received a two-dose primary vaccination series of mRNA-1273 (100 μg in each dose), a first booster dose of 50 μg of mRNA-1273, and a second booster dose of 50 μg of mRNA-1273.214 (Fig. S2 in the Supplementary Appendix). Panel A shows the results in the participants regardless of previous SARS-CoV-2 infection; Panel B, the results in those without previous infection; and Panel C, the results in those with previous infection. The spike mutations for the ancestral SARS-CoV-2 D614G strain and the omicron subvariants are provided in Figure S3. The geometric mean titers (GMTs) of neutralizing antibodies at a 50% inhibitory dilution (ID50) were assessed at baseline (before receipt on the day of the second booster) and at day 29 after receipt of the second booster dose. The ID50 GMTs at baseline and at day 29 and the factor increases in the ID50 GMTs at day 29 relative to baseline values are provided. The 95% confidence intervals (indicated by 𝙸 bars) were calculated on the basis of the t-distribution of the log-transformed GMT values and were then back-transformed to the original scale for presentation. The lower limits of quantitation for the pseudovirus neutralization assay (PsVNA) were ID50 GMTs of 18.5 for the ancestral SARS-CoV-2 D614G strain and 19.9 for the omicron subvariant BA.1, and the upper limits of quantitation were 45,118 and 15,503, respectively. The limit of detection of the PsVNA assay for the omicron subvariants BA.4 or BA.5 and BA.2.75 was 10; values below the limit of detection were assigned a value of 5.
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References

    1. Chalkias S, Harper C, Vrbicky K, et al. A bivalent omicron-containing booster vaccine against Covid-19. N Engl J Med 2022;387:1279-1291. - PMC - PubMed
    1. Shen X, Chalkias S, Feng J, et al. Neutralization of SARS-CoV-2 omicron BA.2.75 after mRNA-1273 vaccination. N Engl J Med 2022;387:1234-1236. - PMC - PubMed
    1. Shen X, Tang H, McDanal C, et al. SARS-CoV-2 variant B.1.1.7 is susceptible to neutralizing antibodies elicited by ancestral spike vaccines. Cell Host Microbe 2021;29(4):529-539.e3. - PMC - PubMed

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