Genetically Encoded Phosphine Ligand for Metalloprotein Design

J Am Chem Soc. 2022 Dec 21;144(50):22831-22837. doi: 10.1021/jacs.2c09683. Epub 2022 Nov 23.


Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ligands
  • Metalloproteins* / genetics
  • Metalloproteins* / metabolism
  • Palladium
  • Phosphines*


  • Metalloproteins
  • Ligands
  • phosphine
  • Phosphines
  • Palladium