Dynamic analysis of the microbial communities and metabolome of healthy banana rhizosphere soil during one growth cycle

PeerJ. 2022 Nov 18;10:e14404. doi: 10.7717/peerj.14404. eCollection 2022.

Abstract

Background: The banana-growing rhizosphere soil ecosystem is very complex and consists of an entangled network of interactions between banana plants, microbes and soil, so identifying key components in banana production is difficult. Most of the previous studies on these interactions ignore the role of the banana plant. At present, there is no research on the the micro-ecological environment of the banana planting growth cycle.

Methods: Based on high-throughput sequencing technology and metabolomics technology, this study analyzed the rhizosphere soil microbial community and metabolic dynamics of healthy banana plants during one growth cycle.

Results: Assessing the microbial community composition of healthy banana rhizosphere soil, we found that the bacteria with the highest levels were Proteobacteria, Chloroflexi, and Acidobacteria, and the dominant fungi were Ascomycota, Basidiomycota, and Mortierellomycota. The metabolite profile of healthy banana rhizosphere soil showed that sugars, lipids and organic acids were the most abundant, accounting for about 50% of the total metabolites. The correlation network between fungi and metabolites was more complex than that of bacteria and metabolites. In a soil environment with acidic pH, bacterial genera showed a significant negative correlation with pH value, while fungal genera showed no significant negative correlation with pH value. The network interactions between bacteria, between fungi, and between bacteria and fungi were all positively correlated.

Conclusions: Healthy banana rhizosphere soil not only has a stable micro-ecology, but also has stable metabolic characteristics. The microorganisms in healthy banana rhizosphere soil have mutually beneficial rather than competitive relationships.

Keywords: Healthy banana planting; Microbial interactions; Soil metabolomics; Soil microbial diversity; Soil microecology.

Grant support

This work was supported by the Bagui Scholars Project of Guangxi Zhuang Autonomous Region, the Guangxi Key R&D Program (2021AB26001), and the Central Government Guides Local Science and Technology Development funds (2020ZYZX3027). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.