Bacterial coculture enhanced Cd sorption and As bioreduction in co-contaminated systems

Huaiting Liu; Zhiqi Hong; Jiahui Lin; Dan Huang; Lena Q Ma; Jianming Xu; Zhongmin Dai

doi:10.1016/j.jhazmat.2022.130376

Bacterial coculture enhanced Cd sorption and As bioreduction in co-contaminated systems

J Hazard Mater. 2023 Feb 15;444(Pt A):130376. doi: 10.1016/j.jhazmat.2022.130376. Epub 2022 Nov 11.

Authors

Huaiting Liu¹, Zhiqi Hong², Jiahui Lin¹, Dan Huang³, Lena Q Ma¹, Jianming Xu⁴, Zhongmin Dai⁵

Affiliations

¹ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
² Agricultural Experiment Station, Zhejiang University, Hangzhou 310058, China.
³ School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
⁴ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; The Rural Development Academy at Zhejiang University, Zhejiang University, Hangzhou 310058, China.
⁵ Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; The Rural Development Academy at Zhejiang University, Zhejiang University, Hangzhou 310058, China. Electronic address: zmdai@zju.edu.cn.

PMID: 36423454
DOI: 10.1016/j.jhazmat.2022.130376

Abstract

The bacterial interactions that regulate Cd sorption and As bioreduction in co-contaminated systems are poorly understood. We isolated two bacterial strains, i.e., Pseudomonas aeruginosa and Bacillus licheniformis from a Cd and As co-contaminated soil and compared the effects of monoculture and coculture on microbial Cd sorption and As bioreduction efficiency in the media with different Cd (0, 0.5, 5, 10, 50, 100 mg/L) and As(Ⅴ) (0, 90 mg/L) concentrations. Compared with monoculture, the bacterial coculture increased the Cd sorption efficiency by up to 32% and the As bioreduction (As(Ⅴ) to As(Ⅲ)) efficiency by up to 28%, associated with the increased abundance of As reduction gene arsB. Based on SEM-TEM and metabolomics, the enhanced efficiency was attributed to bacterial interactions, supported by the differential secretion of extracellular polymeric substances. Notably, the differential lipids and lipid-like molecules, and organoheterocyclic compounds resulted from bacterial interactions compared to monoculture exhibited the highest Cd sorption and As bioreduction. The increased efficiencies by bacterial coculture were verified by soil incubation experiments. These results provide insight on applying specific bacterial coculture and their metabolites to enhance Cd sorption and As bioreduction in effective and sustainable remediation of co-contaminated environments.

Keywords: Arsenic; Cadmium; Co-contamination; Coculture; Interaction; Remediation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria*
Cadmium*
Coculture Techniques
Extracellular Polymeric Substance Matrix
Soil

Substances

Cadmium
Soil