Immunodiagnosis of infection with Schistosoma mansoni: enzyme-linked immunosorbent assay for detection of antibody to circulating antigen

Proc Natl Acad Sci U S A. 1978 Nov;75(11):5715-7. doi: 10.1073/pnas.75.11.5715.


A circulating antigen, a negatively charged polysaccharide from the trematode Schistosoma mansoni, was noncovalently bound to the surface of poly(L-lysine)-coated wells in polystyrene trays, which were then used in a micro-enzyme-linked immunosorbent assay (ELISA) test. The method provides an immunodiagnostic test for schistosomiasis of exceptional sensitivity with a high degree of specificity. Comparison of Bell egg counts and ELISA titers revealed a good correlation (r congruent to 0.80) in young individuals with low to moderate worm burdens, but this relationship was less marked in older individuals or those with high egg counts.

MeSH terms

  • Animals
  • Guinea Pigs
  • Humans
  • Immunoenzyme Techniques
  • Immunosorbent Techniques
  • Schistosoma mansoni / immunology*
  • Schistosoma mansoni / pathogenicity
  • Schistosomiasis / diagnosis*