Structural characterization of a new high molecular weight polysaccharide from jujube fruit

Xiaolong Ji; Zhiwen Wang; Xiyu Hao; Yingying Zhu; Yan Lin; Guoli Li; Xudan Guo

doi:10.3389/fnut.2022.1012348

Structural characterization of a new high molecular weight polysaccharide from jujube fruit

Front Nutr. 2022 Nov 17:9:1012348. doi: 10.3389/fnut.2022.1012348. eCollection 2022.

Authors

Xiaolong Ji¹, Zhiwen Wang^{1

2}, Xiyu Hao³, Yingying Zhu¹, Yan Lin¹, Guoli Li², Xudan Guo⁴

Affiliations

¹ College of Food and Bioengineering, Zhengzhou University of Light Industry, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, China.
² Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing Three Gorges Medical College, Chongqing, China.
³ Heilongjiang Feihe Dairy Co., Ltd., Beijing, China.
⁴ Basic Medical College, Hebei University of Chinese Medicine, Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation, Hebei TCM Formula Preparation Technology Innovation Center, Shijiazhuang, China.

Abstract

From Ziziphus Jujuba cv. Muzao fruit, a new polysaccharide (PZMP3-1) with high molecular weight was isolated. Constructional characterization revealed that PZMP3-1 comprized 2.56 rhamnose, 7.70 arabinoses, 3.73 galactose, and 6.73 galactose, and it has a 241 kDa average molecular weight. The principal structural components of PZMP3-1 were 1,2,4 and 1,4-linked GalpA, 1,4-linked Galp, 1,3 and 1,5-linked Araf, and 1-linked Rhap based on methylation and nuclear magnetic resonance spectroscopy (NMR) analyses. X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), atomic force microscopy (AFM), and scanning electron microscopy (SEM) structural analysis of PZMP3-1 revealed a tangled and branching pattern. Overall, these structural results suggested that PZMP3-1 could have unique bioactivities and be widely used in nutritional supplements.

Keywords: GC-MS; NMR; high-molecular-weight polysaccharide; jujube; structural characterization.