A 3D view of how enteroviruses hijack autophagy

Selma Dahmane; Kasturika Shankar; Lars-Anders Carlson

doi:10.1080/15548627.2022.2153572

A 3D view of how enteroviruses hijack autophagy

Autophagy. 2023 Jul;19(7):2156-2158. doi: 10.1080/15548627.2022.2153572. Epub 2022 Dec 5.

Authors

Selma Dahmane^{1

2

3

4}, Kasturika Shankar^{1

2

3

4}, Lars-Anders Carlson^{1

2

3

4}

Affiliations

¹ Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
² Wallenberg Centre for Molecular Medicine, Umeå University, Umeå, Sweden.
³ The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden.
⁴ Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.

Abstract

Viruses are masters at using cellular pathways to aid their replication. Cryo-electron tomography of poliovirus-infected cells revealed how it utilizes macroautophagy to its advantage. Assembly of these non-enveloped virions takes place directly on membranes and requires PIK3C3/VPS34 activity to be completed, whereas the canonical autophagy inducer ULK1 restricts virus assembly. The tomograms further revealed that enterovirus-induced autophagy is selective for RNA-loaded virions, which may help ensure maximum infectivity of the virus-laden vesicles released through secretory autophagy.

Keywords: Autophagy; cryo-EM; cryo-electron tomography; enteroviruses; membrane trafficking; membranes; poliovirus; virology; virus replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy
Enterovirus*
Poliovirus*
Virus Replication

Grants and funding

This work was supported by the European Commission [795892]; Human Frontier Science Program [CDA00047/2017-C]; Vetenskapsrådet [2021–01145]; Vetenskapsrådet [2018–05851].