Acrylamide is a compound that occurs with high temperature during food processing and causes oxidative damage. Recently, the importance of antioxidative components is increasing to prevent oxidative damage. Lactoferrin is an antioxidant protein mainly found in milk. Therefore, the aim of this study is to determine the dose-dependent protective effects of lactoferrin on oxidative damage caused by acrylamide. In this study, HepG2 cell lines were treated with lactoferrin doses (0, 25, 50, 100µM) and half maximal inhibitory concentration of acrylamide. After 24 hours malondialdehyde, superoxide dismutase, catalase and glutathione reductase levels were measured. Acrylamide significantly increased malondialdehyde levels in HepG2 cells compared to the control group; however, catalase, superoxide dismutase and glutathione reductace significantly reduced. On the other hand, added lactoferrin doses (50-100µM) significantly reduced lipid peroxidation levels. Besides, it was found that glutathione reductase, catalase and superoxide dismutase levels significantly increased. As a result, the protective effect of lactoferrin against the oxidative damage caused by acrylamide in HepG2 cells was determined. This effect is thought to be due to the antioxidant capacity of lactoferrin. In this context, it is recommended that more studies are carried out on the mechanism of action of lactoferrin on oxidative stress caused by acrylamide.