Microbial community profiling in bio-stimulated municipal solid waste for effective removal of organic pollutants containing endocrine disrupting chemicals

Pooja Sharma; Ashok Kumar Nadda; Sunil Kumar

doi:10.1016/j.micres.2022.127273

Microbial community profiling in bio-stimulated municipal solid waste for effective removal of organic pollutants containing endocrine disrupting chemicals

Microbiol Res. 2023 Feb:267:127273. doi: 10.1016/j.micres.2022.127273. Epub 2022 Nov 25.

Authors

Pooja Sharma¹, Ashok Kumar Nadda², Sunil Kumar³

Affiliations

¹ Waste Re-processing Division, CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, Maharashtra, India.
² Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh 173 234, India.
³ Waste Re-processing Division, CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, Maharashtra, India. Electronic address: sunil_neeri@yahoo.co.in.

PMID: 36481500
DOI: 10.1016/j.micres.2022.127273

Abstract

The study was aimed to improve the degradation of organic pollutants in municipal solid waste (MSW) through the bio-stimulation process. The results showed that the physico-chemical properties of MSW (control) had a high value of pH (9.2 ± 0.02); total suspended solids (TSS: 1547 ± 23 mg/kg^-1), and total dissolved solids (TDS:76 ± 0.67 mg/kg^-1). After the biostimulation process (biostimulated MSW), the physico-chemical parameters of MSW were reduced as pH (7.1 ± 0.01); TSS (41 ± 0.01 mg/kg^-1), and TDS (789 ± 03 mg/kg^-1). Furthermore, the major organic pollutants detected from MSW by gas chromatography-mass spectroscopy (GC-MS) analysis at different retention time (RT) were hexadecane (RT-8.79); pentadecane (RT-9.36); and hexasiloxane (RT-9.43) while these organic pollutants were degraded after the biostimulation process. The whole-genome metagenome sequencing size (%) analyses showed major groups of bacteria (40.82%) followed by fungi (0.05%), virus (0.0032%), and archaea (0.0442%) in MSW. The species richness and evenness of the microbial community were decreased substantially due to the biostimulation treatment. The total number of genes in the biostimulated MSW (PS-3_11267) sample were 465302 whereas the number of genes in the control MSW (PS-4_11268) sample were 256807. Furthermore, the biostimulated MSW (PS-3_11267) aligned the reads to bacteria (19502525), fungi (40030), virus (3339), and archaea (12759) genomes whereas the control sample (PS-4_11268) aligned the reads to bacteria (17057259), fungi (19148), virus (1335), and archaea (18447) genomes. Moreover, the relative abundance at genus level in biostimulated MSW (PS-3_11267) (Ochrobactrum and Phenylobacterium), phylum; (Proteobacteria and Actinobacteria), and species (Chthoniobacter flavus and Vulgatibacter incomptus) level was the most abundant. The results provided valuable information regarding the degradation of organic pollutants in MSW by microbial communities through biostimulation for the prevention of soil pollution and health protection.

Keywords: Biostimulation; Community dynamics; Endocrine-disrupting chemicals; Microbial community; Organic pollutants.

MeSH terms

Archaea / genetics
Bacteria / genetics
Endocrine Disruptors* / analysis
Environmental Pollutants* / analysis
Microbiota*
Solid Waste / analysis

Substances

Endocrine Disruptors
Environmental Pollutants
Solid Waste