Fabrication of gentamicin loaded Col-I/HA multilayers modified titanium coatings for prevention of implant infection

Le Ma; Jiajia Zong; Xiaowei Xun; Xiaoming Hu; Zejing Chen; Quanchao Zhang; Mengxia Peng; Botao Song; Haiyong Ao

doi:10.3389/fchem.2022.1019332

Fabrication of gentamicin loaded Col-I/HA multilayers modified titanium coatings for prevention of implant infection

Front Chem. 2022 Nov 22:10:1019332. doi: 10.3389/fchem.2022.1019332. eCollection 2022.

Authors

Le Ma¹, Jiajia Zong¹, Xiaowei Xun¹, Xiaoming Hu¹, Zejing Chen¹, Quanchao Zhang¹, Mengxia Peng¹, Botao Song², Haiyong Ao¹

Affiliations

¹ Jiangxi Key Laboratory of Nanobiomaterials, School of Materials Science and Engineering, East China Jiaotong University, Nanchang, China.
² Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi, China.

Abstract

In this study, gentamicin loaded collagen I/hyaluronic acid multilayers modified titanium coating (TC-AA(C/H)₆-G) was fabricated via a layer-by-layer (LBL) covalent immobilization method. The drug releasing properties of collagen I/Hyaluronic acid (Col-I/HA) multilayers and the effect of loaded gentamicin on the antibacterial properties and cytocompatibility of modified TC were investigated. The gentamicin release assay indicated that the Col-I/HA multilayers modified TC exhibited agreeable drug-loading amount (537.22 ± 29.66 µg of gentamicin) and controlled-release performance (240 h of sustained release time). TC-AA(C/H)₆-G revealed satisfactory antibacterial activity and inhibited the colonization and biofilm formation of S. aureus. Fortunately, the functions of hMSCs on TC-AA(C/H)₆-G did not affected by the loaded gentamicin, and TC-AA(C/H)₆-G could improve the adhesion, proliferation and osteogenic differentiation of cells, as well as TC-AA(C/H)₆. In vivo animal study indicated that TC-AA(C/H)₆-G could effectively control intramedullary cavity infection caused by S. aureus and prevent bone destruction.

Keywords: Col-I/HA multilayers; cytocompatibility; drug-release; prevention infection; titanium coatings.