Genome-wide identification and expression analysis of PIN gene family under phytohormone and abiotic stresses in Vitis Vinifera L

Huimin Gou; Guojie Nai; Shixiong Lu; Weifeng Ma; Baihong Chen; Juan Mao

doi:10.1007/s12298-022-01239-8

Genome-wide identification and expression analysis of PIN gene family under phytohormone and abiotic stresses in Vitis Vinifera L

Physiol Mol Biol Plants. 2022 Oct;28(10):1905-1919. doi: 10.1007/s12298-022-01239-8. Epub 2022 Nov 5.

Authors

Huimin Gou¹, Guojie Nai¹, Shixiong Lu¹, Weifeng Ma¹, Baihong Chen¹, Juan Mao¹

Affiliation

¹ College of Horticulture, Gansu Agricultural University, Lanzhou, 730070 Gansu Province People's Republic of China.

Abstract

The auxin efflux transport proteins PIN-formed (PIN) has wide adaptability to hormone and abiotic stress, but the response mechanism of PINs in grape remains unclear. In this study, 12 members of VvPINs were identified and distributed on 8 chromosomes. The PIN genes of five species were divided into two subgroups, and the similarity of exons/introns and motifs of VvPIN genes were found in the same subgroup. Meanwhile, according to the examination of conserved motifs, the motif 3 included the conserved structure NPNTY. The promoter region of VvPIN gene family contained various cis-acting elements, which were related to light, abiotic stress, and hormones which are essential for growth and development. Additionally, VvPIN1, VvPIN9, and VvPIN11 proteins simultaneously interacted with the ARF, ABC, PINOID, GBF1, and VIT_08s0007g09010. The results of qRT-PCR revealed that the majority of the VvPINs were highly induced by NAA, GA3, ABA, MeJA, SA, NaCl, low-temperature (4 ℃), and PEG treatments, and the results were consistent with the prediction of the cis-acting elements. Moreover, the expression profile and quantitative real-time PCR (qRT-PCR) demonstrated that VvPIN genes were expressed in roots, stems, and leaves. The subcellular localization of VvPIN1 in Nicotiana benthamiana revealed that VvPIN1 was localized at the plasma membrane. Collectively, this study revealed that PIN genes could respond to various abiotic stresses, and provided a framework for regulating the expression of PIN genes to enhance the resistance of the grape.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-022-01239-8.

Keywords: Abiotic stresses; Auxin; Grape; PIN gene family; Phytohormone; Subcellular proteins.

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