Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

Virol Sin. 2023 Feb;38(1):165-170. doi: 10.1016/j.virs.2022.12.001. Epub 2022 Dec 6.

¹ State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China.
² Laboratory Department of the First Medical Center, Chinese PLA General Hospital, Beijing, 100850, China.
³ State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: jiangtao@bmi.ac.cn.
⁴ State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: kangxiaoping@bmi.ac.cn.

Abstract

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Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
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The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA.
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No cross-reaction was found in 14 rash and fever-associated viruses.
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The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.