miRNA Theranostic Nanoparticles Promote Pancreatic Beta Cell Proliferation in Type 1 Diabetes Model

Saumya Nigam; Anna Moore; Ping Wang

doi:10.1007/978-1-0716-2807-2_15

miRNA Theranostic Nanoparticles Promote Pancreatic Beta Cell Proliferation in Type 1 Diabetes Model

Methods Mol Biol. 2023:2592:207-218. doi: 10.1007/978-1-0716-2807-2_15.

Authors

Saumya Nigam^{1

2}, Anna Moore^{3

4}, Ping Wang^{5

6}

Affiliations

¹ Precision Health Program, Michigan State University, East Lansing, MI, USA.
² Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.
³ Precision Health Program, Michigan State University, East Lansing, MI, USA. moorea57@msu.edu.
⁴ Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA. moorea57@msu.edu.
⁵ Precision Health Program, Michigan State University, East Lansing, MI, USA. wangpin4@msu.edu.
⁶ Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA. wangpin4@msu.edu.

PMID: 36507996
DOI: 10.1007/978-1-0716-2807-2_15

Abstract

Type 1 diabetes (T1D) is a chronic autoimmune disorder which affects the insulin-producing beta cells in the pancreas. A variety of strategies, namely, insulin replacement therapy, engineered vaccines, immunomodulators, etc., have been explored to correct this condition. Recent studies have attributed the development of T1D to the anomalous expression of microRNAs in the pancreatic islets. Here, we describe the protocol for the development of a theranostic approach to modify the expression of aberrant miRNAs. The MRI-based nanodrug consists of superparamagnetic iron oxide nanoparticles conjugated to microRNA-targeting oligonucleotides that can promote proliferation of pancreatic beta cells in a mouse model of T1D. This theranostic approach can successfully serve as a potential therapeutic approach for the targeted treatment of T1D with minimal side effects.

Keywords: Magnetic resonance imaging; MicroRNA; Nanoparticles; Pancreatic beta cell; Theranostics; Type 1 diabetes.

MeSH terms

Animals
Cell Proliferation
Diabetes Mellitus, Type 1* / genetics
Diabetes Mellitus, Type 1* / metabolism
Diabetes Mellitus, Type 1* / therapy
Insulin / metabolism
Insulin-Secreting Cells* / metabolism
Mice
MicroRNAs* / genetics
MicroRNAs* / metabolism

Substances

MicroRNAs
Insulin