Gut microbiota is associated with response to 131I therapy in patients with papillary thyroid carcinoma

Abstract

Purpose: Radioactive iodine (¹³¹I) therapy is a conventional post-surgery treatment widely used for papillary thyroid carcinoma (PTC). Since ¹³¹I is orally administered, we hypothesize that it may affect gut microbiome. This study aims to investigate alterations of intestinal microbiome caused by ¹³¹I therapy in PTC patients and explore its association with response to ¹³¹I therapy.

Methods: Fecal samples of 60 PTC patients pre- and post-¹³¹I therapy were collected to characterize the ¹³¹I therapy-induced gut microbiota alterations using 16S rRNA gene sequencing. According to the inclusion criteria, sequence data of 40 out of the 60 patients, divided into excellent response (ER) group and non-excellent response (NER) group, were recruited to investigate the possible connection between gut microbiota and response to ¹³¹I therapy. Multivariate binary logistic regression was employed to construct a predictive model for response to ¹³¹I therapy.

Results: Microbial richness, diversity, and composition were tremendously altered by ¹³¹I therapy. A significant decline of Firmicutes to Bacteroides (F/B) ratio was observed post-¹³¹I therapy. ¹³¹I therapy also led to changes of gut microbiome-related metabolic pathways. Discrepancies in β diversity were found between ER and NER groups both pre- and post-¹³¹I therapy. Furthermore, a predictive model for response to ¹³¹I therapy with a p value of 0.003 and an overall percentage correct of 80.0% was established, with three variables including lymph node metastasis, relative abundance of g_Bifidobacterium and g_Dorea. Among them, g_Dorea was identified to be an in independent predictor of response to ¹³¹I therapy (p = 0.04).

Conclusion: For the first time, the present study demonstrates the gut microbial dysbiosis caused by ¹³¹I therapy in post-surgery PTC patients and reveals a previously undefined role of gut microbiome as predictor for ¹³¹I ablation response. G_Dorea and g_Bifidobacterium may be potential targets for clinical intervention to improve response to ¹³¹I in post-operative PTC patients.

Trial registration: ChiCTR2100048000. Registered 28 June 2021.

Keywords: 131I response; 131I therapy; 16s rRNA; Gut microbiota; Predictive model; Radioactive iodine therapy.