The polarization perception sensitivity of the human eyes affects the perceived polarized image quality. In this paper, we used polarized spatiotemporal structured images to develop a spatiotemporal age mapping of the polarization perception of human eyes. We built an optical modulation transfer function mathematical model of the aging human eyes with spatiotemporal frequency domains and introduced the Stokes vector to analyze the polarized images. The proposed model provides a testing method based on a set of polarization images with spatiotemporal frequencies varying according to the perception of differently aged viewers. Then, we experimentally validated the proposed model by performing polarization perception tests on a group of volunteers. The test method has the diagnostic potential to confirm the health of human eyes and identify potential age-related macular diseases.