An animal model of EPO-induced abdominal aortic aneurysm in WT and Apoe-/- mice

Meng Zhang; Wenhai Sui; Meng Zhang; Yun Zhang; Cheng Zhang

doi:10.1016/j.xpro.2022.101929

An animal model of EPO-induced abdominal aortic aneurysm in WT and Apoe^-/- mice

STAR Protoc. 2023 Mar 17;4(1):101929. doi: 10.1016/j.xpro.2022.101929. Epub 2022 Dec 15.

Authors

Meng Zhang¹, Wenhai Sui¹, Meng Zhang², Yun Zhang³, Cheng Zhang⁴

Affiliations

¹ Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.
² Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China. Electronic address: zhangmeng@sdu.edu.cn.
³ Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China. Electronic address: zhangyun@sdu.edu.cn.
⁴ Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China; Cardiovascular Disease Research Center of Shandong First Medical University, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. Electronic address: zhangc@sdu.edu.cn.

Abstract

Abdominal aortic aneurysm (AAA) is a potentially fatal vascular disease, but the underlying mechanisms remain obscure. Here, we provide a protocol using erythropoietin (EPO) to induce the formation of AAA in both wild-type (WT) and apolipoprotein E (Apoe^-/-) mice. We describe the dose, manner, and timing of EPO administration. We also detail mice dissection, aorta isolation, and histological analysis. The animal model of EPO-induced AAA provides a useful tool for exploring the mechanism of AAA in experimental studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).¹.

Keywords: Health Sciences; Microscopy; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aorta, Abdominal / pathology
Aortic Aneurysm, Abdominal* / chemically induced
Apolipoproteins E / adverse effects
Apolipoproteins E / genetics
Disease Models, Animal
Erythropoietin* / adverse effects
Mice

Substances

Apolipoproteins E
Erythropoietin