A polyethyleneimine-functionalized polymer substrate polar stationary phase

Yufeng Shen; Huiliang Geng; Feifang Zhang; Zongying Li; Bingcheng Yang

doi:10.1016/j.chroma.2022.463711

A polyethyleneimine-functionalized polymer substrate polar stationary phase

J Chromatogr A. 2023 Jan 25:1689:463711. doi: 10.1016/j.chroma.2022.463711. Epub 2022 Dec 13.

Authors

Yufeng Shen¹, Huiliang Geng¹, Feifang Zhang², Zongying Li¹, Bingcheng Yang³

Affiliations

¹ Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East-China University of Science and Technology, 130 Meilong RD, Pharmacy School, Shanghai 200237, China.
² Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East-China University of Science and Technology, 130 Meilong RD, Pharmacy School, Shanghai 200237, China. Electronic address: zhangff@ecust.edu.cn.
³ Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East-China University of Science and Technology, 130 Meilong RD, Pharmacy School, Shanghai 200237, China. Electronic address: bcyang@ecust.edu.cn.

PMID: 36586280
DOI: 10.1016/j.chroma.2022.463711

Abstract

A polyethyleneimine (PEI)-functionalized polymer substrate polar stationary phase was prepared for hydrophilic interaction chromatography (HILIC) by grafting PEI onto poly(styrene-divinylbenzene) (PS-DVB) microspheres. The phase shows a U-shape retention profile and it exhibits typical hydrophilic characteristic when the organic solvent fraction in the mobile phase is > 60%. Hydrogen bonding, anion exchange, and hydrophobic interaction are involved in the retention mechanism. Good separation and unique selectivity for acidic, basic and neutral polar analytes were achieved. It showed extremely low column bleed (comparable to that of blank) under gradient elution mode (even to 50% fraction of water) and wide pH tollerance range (at least 1-13).

Keywords: Bleed; Hydrophilic interaction liquid chromatography; Polyethyleneimine; Polymer; Stationary phase.

MeSH terms

Chromatography, Liquid / methods
Chromatography, Reverse-Phase / methods
Hydrophobic and Hydrophilic Interactions
Polyethyleneimine* / chemistry
Polymers*
Solvents

Substances

Polyethyleneimine
Polymers
Solvents