Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

Makoto Ito; Yohei Ishibashi; Takashi Watanabe; Jun Iwaki; Toyohisa Kurita; Nozomu Okino

doi:10.1007/978-1-0716-2910-9_18

Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

Methods Mol Biol. 2023:2613:229-256. doi: 10.1007/978-1-0716-2910-9_18.

Authors

Makoto Ito¹, Yohei Ishibashi², Takashi Watanabe^{2

3}, Jun Iwaki⁴, Toyohisa Kurita⁵, Nozomu Okino⁶

Affiliations

¹ Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. makotoi@agr.kyushu-u.ac.jp.
² Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
³ Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
⁴ Tokyo Chemical Industry Co., Ltd., Tokyo, Japan.
⁵ TAKARA BIO INC., Shiga, Japan.
⁶ Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. nokino@agr.kyushu-u.ac.jp.

PMID: 36587083
DOI: 10.1007/978-1-0716-2910-9_18

Abstract

Microbial glycosphingolipid (GSL)-degrading enzymes with unique specificity are useful tools for GSL research. On the other hand, some microbial glycolipids, not only GSLs but also steryl glucosides, are closely related to pathogenicity, and, thus, the metabolism of microbial glycolipids is attracting attention as a target for antibiotics. This chapter describes the assays and utilization of microbial enzymes useful for glycolipid research and those involved in pathogenicity or host immune reactions.

Keywords: Bacteria; Fungi; Glycohydrolase; Glycolipid; Glycosyltransferase; N-deacylase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / metabolism
Fungi / metabolism
Glycolipids* / metabolism
Glycosphingolipids* / metabolism

Substances

Glycolipids
Glycosphingolipids

Supplementary concepts

Neuraminidase deficiency with beta-galactosidase deficiency