Fluvoxamine ameliorates oxidative stress and inflammation induced by bile-duct ligation in male rats

Zahra Barmoudeh; Hossein Sadeghi; Izadpanah Gheitasi; Bahman Khalvati; Navid Omidifar; Mahdokht Azizi; Zahra Moslemi; Jafar Nikbakht; Amir Hossein Doustimotlagh

doi:10.1016/j.heliyon.2022.e12344

Fluvoxamine ameliorates oxidative stress and inflammation induced by bile-duct ligation in male rats

Heliyon. 2022 Dec 15;8(12):e12344. doi: 10.1016/j.heliyon.2022.e12344. eCollection 2022 Dec.

Authors

Zahra Barmoudeh¹, Hossein Sadeghi², Izadpanah Gheitasi², Bahman Khalvati², Navid Omidifar³, Mahdokht Azizi², Zahra Moslemi¹, Jafar Nikbakht², Amir Hossein Doustimotlagh²

Affiliations

¹ Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
² Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
³ Biotechnology Research Center, Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Introduction: Cholestasis is a disorder that the bile ducts were narrowed and bile acids are not released simply. Bile acids-induced liver damage is exacerbated by inflammation and oxidative stress. The goal of the current study was to investigate the protective impacts of fluvoxamine (Flu) on oxidant-antioxidant balance and inflammatory cytokines in the bile duct ligated (BDL) rats.

Methods: Thirty-two male rats were arbitrarily allocated in 4 groups; sham-control (SC), SC+ 150 mg/kg Flu (SCF), bile duct ligation (BDL), and BDL+ 150 mg/kg Flu (BDLF). The rats received distilled water and Flu orally for one week. Biochemical analysis, hematoxylin and eosin staining, as well as oxidant/antioxidant status were evaluated. Also, the mRNA expression of TGF-β1, IL-1, TNF-α, and α-SMA were determined.

Results: The findings indicated serum values of ALT, total bilirubin, and ALP slightly declined in the BDL + Flu group in contrast to BDL rats. The plasma protein carbonyl and inflammatory markers were markedly increased in the BDL group in contrast with SC group (P ≤ 0.05). Treatment with Flu in BDL rats markedly reduced the values of hepatic nitric oxide metabolite and malondialdehyde, plasma protein carbonyl, as well as TNF-α mRNA level (P ≤ 0.05). Histological parameters were improved in the BDL + Flu group in comparison to BDL merely rats.

Conclusion: It seems that Flu declined oxidative stress probably by inhibiting lipid peroxidation, protein oxidation, and nitric oxide formation. Also, it reduced inflammation by decreasing TNF-α mRNA expression.

Keywords: Antioxidant; Cholestasis; Fluvoxamine; Inflammation; Oxidative stress.