Labeling and analyzing lipid droplets in mouse muscle stem cells

STAR Protoc. 2022 Dec 16;3(4):101849. doi: 10.1016/j.xpro.2022.101849. Epub 2022 Nov 19.


Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining of freshly isolated MuSCs, in vitro cultured myoblasts, and single myofibers to label lipid droplets and subsequent analysis and quantification of fluorescence signals. This protocol can be modified to stain lipid droplets in other cell types of interest. For complete details on the use and execution of this protocol, please refer to Yue et al. (2022).1.

Keywords: Cell Biology; Cell culture; Flow Cytometry/Mass Cytometry; Microscopy; Molecular/Chemical Probes; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Fluorescence
  • Lipid Droplets*
  • Mice
  • Muscle Fibers, Skeletal
  • Muscle, Skeletal*
  • Stem Cells