Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells

Yukiko Tando; Yasuhisa Matsui

doi:10.1016/j.xpro.2022.101912

Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells

STAR Protoc. 2022 Dec 16;3(4):101912. doi: 10.1016/j.xpro.2022.101912. Epub 2022 Dec 9.

Authors

Yukiko Tando¹, Yasuhisa Matsui²

Affiliations

¹ Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan; Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan; Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan. Electronic address: yukiko.tando.a2@tohoku.ac.jp.
² Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan; Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan; Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japan. Electronic address: yasuhisa.matsui.d3@tohoku.ac.jp.

Abstract

It is now recognized that maternal environmental factors, including chemical exposure and nutritional conditions, alter DNA methylation patterns in fetal germ cells, subsequently affecting germ cell development as well as offspring phenotypes. Here, we describe steps for detecting DNA methylation changes in mouse germ cells isolated from both embryonic and spermatogenic stages after maternal exposure to a chemical compound. For complete details on the use and execution of this protocol, please refer to Tando et al. (2021).¹.

Keywords: Developmental biology; Metabolism; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Methylation*
Female
Germ Cells
Humans
Male
Maternal Exposure* / adverse effects
Mice
Reproduction
Spermatogenesis / genetics