Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells

STAR Protoc. 2022 Dec 16;3(4):101872. doi: 10.1016/j.xpro.2022.101872. Epub 2022 Nov 7.


This protocol presents the use of SARS-CoV-2 isolates to infect human kidney organoids, enabling exploration of the impact of SARS-CoV-2 infection in a human multicellular in vitro system. We detail steps to generate kidney organoids from human pluripotent stem cells (hPSCs) and emulate a diabetic milieu via organoids exposure to diabetogenic-like cell culture conditions. We further describe preparation and titration steps of SARS-CoV-2 virus stocks, their subsequent use to infect the kidney organoids, and assessment of the infection via immunofluorescence. For complete details on the use and execution of this protocol, please refer to Garreta et al. (2022).1.

Keywords: Cell Differentiation; Cell culture; Microbiology; Microscopy; Organoids; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • COVID-19*
  • Cell Differentiation
  • Humans
  • Kidney
  • Organoids
  • Pluripotent Stem Cells*
  • SARS-CoV-2