Continuous chromogenic tissue factor assay: comparison to clot-based assays and sensitivity established using pure tissue factor

Thromb Res. 1987 Aug 15;47(4):379-87. doi: 10.1016/0049-3848(87)90453-1.


The continuous chromogenic tissue factor assay has been compared to one-stage and two-stage coagulation assays, and its sensitivity has been established using purified human placental tissue factor. Log-log plots of the clot-times versus A405/min2 are linear over the mutually useful ranges of tissue factor concentration. Using human placental tissue factor reconstituted into phosphatidylserine-phosphatidylcholine (30:70) vesicles, the chromogenic assay quantitatively detected tissue factor in dilutions containing from 3 femtomoles to less than 10 attomoles of protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Coagulation Tests*
  • Brain / metabolism
  • Cattle
  • Chromogenic Compounds / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Methods
  • Placenta / analysis
  • Pregnancy
  • Thromboplastin / analysis*
  • Whole Blood Coagulation Time


  • Chromogenic Compounds
  • Thromboplastin