Fundidesulfovibrio agrisoli sp. nov., A Nitrogen-Fixing Bacterium Isolated from Rice Field

Curr Microbiol. 2023 Jan 7;80(2):68. doi: 10.1007/s00284-022-03140-9.


A strictly anaerobic nitrogen-fixing strain, designated SG106T, was isolated from rice field. The 16S rRNA gene sequence analysis showed that strain SG106T was closely related to the type strain of Fundidesulfovibrio magnetotacticus (97.3%). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (constructed using a concatenated alignment of 117 conserved bacterial single-copy genes with GTDB-Tk) trees, strain SG106T clustered with members of the genus Fundidesulfovibrio. Strain SG106T grew at 20-40 °C and 0-0.4% (w/v) NaCl. Desulfoviridin was found in the strain SG106T. The genomic DNA G + C content of strain SG106T was 66.0%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG106T and the closely related F. magnetotacticus were 78.4% and 21.7%, respectively. Genome analysis showed that strain SG106T encodes genes for nitrogen fixation (nifHDK). Acetylene reduction experiments showed that the nitrogenase activity of strain SG106T could reach 224.7 μmol C2H4 g-1 protein h-1. Based on the above results, strain SG106T represents a novel species of the genus Fundidesulfovibrio, for which the name Fundidesulfovibrio agrisoli sp. nov. is proposed. The type strain is SG106T (= GDMCC 1.3136T = JCM 35588T).

MeSH terms

  • Bacterial Typing Techniques
  • DNA, Bacterial / genetics
  • Fatty Acids*
  • Oryza* / microbiology
  • Phospholipids
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • Fatty Acids
  • RNA, Ribosomal, 16S
  • DNA, Bacterial
  • Phospholipids