Bioprocess development to enhance biomass and lutein production from Chlorella sorokiniana Kh12

Akash Pralhad Vadrale; Cheng-Di Dong; Dibyajyoti Haldar; Chien-Hui Wu; Chiu-Wen Chen; Reeta Rani Singhania; Anil Kumar Patel

doi:10.1016/j.biortech.2023.128583

Bioprocess development to enhance biomass and lutein production from Chlorella sorokiniana Kh12

Bioresour Technol. 2023 Feb:370:128583. doi: 10.1016/j.biortech.2023.128583. Epub 2023 Jan 5.

Authors

Akash Pralhad Vadrale¹, Cheng-Di Dong², Dibyajyoti Haldar³, Chien-Hui Wu⁴, Chiu-Wen Chen², Reeta Rani Singhania⁵, Anil Kumar Patel⁶

Affiliations

¹ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
² Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan.
³ Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore 641114, India.
⁴ Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
⁵ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India.
⁶ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India. Electronic address: anilkpatel22@nkust.edu.tw.

PMID: 36610481
DOI: 10.1016/j.biortech.2023.128583

Abstract

Present study focused on optimizing bioprocess condition for microalgal lutein production. From previous baseline yields of biomass (3.46 g/L) and lutein (13.7 mg/g), this study examined few key parameters. The 3X:3X ratio macro- and micronutrients was the most affecting parameter with highest biomass and lutein yields of 4.61 g/L and 14.3 mg/g. Temperature 30 °C enhanced the lutein up to 17.3 mg/g but reduced the biomass to 3 g/L. The light effects study showed 10 k lux was most effective for lutein up to 14 mg/g, and effect of increasing salinity (25-75 %) was detrimental. All the above parameters' optimization resulted in a lipid content of 22.5-26.5 %. A maximum lutein productivity and yield of 0.451 mg/L/d and 65.74 mg/L with a 3X:3X macro- and micronutrient ratio was achieved. The Chlorella sorokiniana Kh12 strain exhibited one of the highest yields among recent reports; hence it could be a source for commercial lutein production.

Keywords: Biorefinery; Chlorella sorokiniana; Light intensity; Lipid; Lutein.

MeSH terms

Biomass
Chlorella*
Lutein
Microalgae*
Temperature

Substances

Lutein