Sensitive biosensors based on topological insulator Bi2Se3 and peptide

Yujiu Jiang; Peng Zhu; Jinge Zhao; Shanshan Li; Yetong Wu; Xiaolu Xiong; Xu Zhang; Yuxiang Liu; Jiangyue Bai; Zihang Wang; Shiqi Xu; Minxuan Wang; Tinglu Song; Zhiwei Wang; Weizhi Wang; Junfeng Han

doi:10.1016/j.aca.2022.340655

Sensitive biosensors based on topological insulator Bi₂Se₃ and peptide

Anal Chim Acta. 2023 Jan 25:1239:340655. doi: 10.1016/j.aca.2022.340655. Epub 2022 Nov 22.

Authors

Yujiu Jiang¹, Peng Zhu¹, Jinge Zhao², Shanshan Li³, Yetong Wu¹, Xiaolu Xiong¹, Xu Zhang¹, Yuxiang Liu¹, Jiangyue Bai¹, Zihang Wang¹, Shiqi Xu¹, Minxuan Wang², Tinglu Song⁴, Zhiwei Wang⁵, Weizhi Wang², Junfeng Han⁶

Affiliations

¹ Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China.
² Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electro-photonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China.
³ Department of Rheumatology, China-Japan Friendship Hospital, Beijing, 100029, China.
⁴ Experimental Centre of Advanced Materials School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, China.
⁵ Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China. Electronic address: zhiweiwang@bit.edu.cn.
⁶ Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China. Electronic address: pkuhjf@bit.edu.cn.

PMID: 36628700
DOI: 10.1016/j.aca.2022.340655

Abstract

In this work, we designed a facile and label-free electrochemical biosensor based on intrinsic topological insulator (TI) Bi₂Se₃ and peptide for the detection of immune checkpoint molecules. With topological protection, Bi₂Se₃ could have robust surface states with low electronic noise, which was beneficial for the stable and sensitive electron transport between electrode and electrolyte interface. The peptides are easily synthesized and chemically modified, and have good biocompatibility and bioavailability, which is a suitable candidate as the recognition units for immune checkpoint molecules. Therefore, the peptide/Bi₂Se₃ was developed as a suitable working electrode for the electrochemical biosensor. The basic performance of the designed peptide/Bi₂Se₃ biosensor was investigated to determine the Anti-HA Tag Antibody and PD-L1 molecules. The linear detection range was from 3.6 × 10^-10 mg mL^-1 to 3.6 × 10^-5 mg mL^-1, and the detection limit was 1.07 × 10^-11 mg mL^-1. Moreover, the biosensor also displayed good selectivity and stability.

Keywords: Anti-HA Tag antibody; Bi(2)Se(3); Electrochemical biosensor; Programmed death-ligand 1; Topological insulator.

MeSH terms

Biological Availability
Biosensing Techniques*
Electrodes
Electron Transport
Immune Checkpoint Proteins*
Peptides*

Substances

Immune Checkpoint Proteins
Peptides