Expeditious fluorimetric detection of bilirubin by simple imidazole derived luminophore and it's pragmatic applicability in spiked biological fluids

Kathiresan Anusuyadevi; Sivan Velmathi

doi:10.1016/j.aca.2022.340678

Expeditious fluorimetric detection of bilirubin by simple imidazole derived luminophore and it's pragmatic applicability in spiked biological fluids

Anal Chim Acta. 2023 Jan 25:1239:340678. doi: 10.1016/j.aca.2022.340678. Epub 2022 Nov 26.

Authors

Kathiresan Anusuyadevi¹, Sivan Velmathi²

Affiliations

¹ Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, 620 015, India.
² Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli, 620 015, India. Electronic address: velmathis@nitt.edu.

PMID: 36628705
DOI: 10.1016/j.aca.2022.340678

Abstract

Bilirubin is an indispensable biomarker for liver diseases. Utilizing organic molecules as sensor platform for effective detection of bilirubin are little. In addition, the reported fluorophores required longer incubation time for detection. Hence, herein we have attempted to design an imidazole derivative 4-(3H-imidazo[4,5-b]pyridin-2-yl)-N,N-diphenylaniline (IMI) from triphenylamine and pyridine units which could detect bilirubin swiftly without any incubation period. IMI manifested an instant quenching of emission in presence of bilirubin with limit of detection (LOD) 11.74 × 10^-6 mol L^-1. The mechanistic aspect of detection involves coexistence of both static and dynamic quenching which was suitably justified. Finally, the pragmatic application of IMI was performed in bio-fluids.

Keywords: Bilirubin; Bio-fluids; FRET; Fluorimetric detection; Imidazole.

MeSH terms

Bilirubin*
Fluorometry
Imidazoles*
Limit of Detection
Spectrometry, Fluorescence / methods

Substances

Bilirubin
Imidazoles