Single-cell multi-omics and lineage tracing to dissect cell fate decision-making

Laleh Haghverdi; Leif S Ludwig

doi:10.1016/j.stemcr.2022.12.003

Single-cell multi-omics and lineage tracing to dissect cell fate decision-making

Stem Cell Reports. 2023 Jan 10;18(1):13-25. doi: 10.1016/j.stemcr.2022.12.003.

Authors

Laleh Haghverdi¹, Leif S Ludwig²

Affiliations

¹ Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany. Electronic address: laleh.haghverdi@mdc-berlin.de.
² Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Berlin, Germany; Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Berlin, Germany. Electronic address: leif.ludwig@bih-charite.de.

Abstract

The concept of cell fate relates to the future identity of a cell, and its daughters, which is obtained via cell differentiation and division. Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. In this perspective, we discuss these approaches with a focus on theoretical concepts and future directions of the field to dissect molecular mechanisms underlying cell fate.

Keywords: cell fate decision; clonal tracking; embryogenesis; haematopoiesis; mathematical and computational models; multi-omics.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Cell Lineage / genetics
Genome
Genomics*
Multiomics*
Single-Cell Analysis

Grants and funding

UM1 HG012076/HG/NHGRI NIH HHS/United States