In-depth interpretation of aptamer-based sensing on electrode: Dual-mode electrochemical-photoelectrochemical sensor for the ratiometric detection of patulin

Shuda Liu; Shuyun Meng; Meng Wang; Wenjia Li; Na Dong; Dong Liu; Yuye Li; Tianyan You

doi:10.1016/j.foodchem.2023.135450

In-depth interpretation of aptamer-based sensing on electrode: Dual-mode electrochemical-photoelectrochemical sensor for the ratiometric detection of patulin

Food Chem. 2023 Jun 1:410:135450. doi: 10.1016/j.foodchem.2023.135450. Epub 2023 Jan 9.

Authors

Shuda Liu¹, Shuyun Meng¹, Meng Wang², Wenjia Li¹, Na Dong¹, Dong Liu³, Yuye Li¹, Tianyan You¹

Affiliations

¹ Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
² Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. Electronic address: wangm@iqstt.cn.
³ Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address: dongliu@ujs.edu.cn.

PMID: 36640656
DOI: 10.1016/j.foodchem.2023.135450

Abstract

Electrochemical aptasensors have been extensively used to quantify food contaminants (e.g., mycotoxin) by using high-affinity aptamer for target recognition. Yet, analytical performance of aptasensors using different aptamers can be varied for the same target. Here, four aptamers with different sequences (i.e., A22, A34, A42, and A45) of patulin (PAT) were selected to estimate sensing behaviors at electrodes with electrochemical (EC) and photoelectrochemical (PEC) assays. Synergistic effect of steric hindrance and electron transfer distance was found to significantly affect EC and PEC response for PAT at aptasensors fabricated with A22, A34, A42, or A45. Eventually, A22 emerged to be the optimal aptamer for aptasensing, despite the highest affinity of A42 to PAT. The A22-based EC-PEC dual-mode ratiometric aptasensor offered a linear range of 50 fg mL^-1 - 500 ng mL^-1 with a detection limit of 30 fg mL^-1 for PAT, and it was applied to apple product (i.e., juice, puree) analysis.

Keywords: Aptamer; Conformation change; Dual-mode detection; Patulin; Ratiometric aptasensor.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Electrochemical Techniques
Electrodes
Gold
Limit of Detection
Malus*
Patulin* / analysis

Substances

Patulin
Aptamers, Nucleotide
Gold